Poster Presentation The Annual Scientific Meeting of the Australian Diabetes Society and the Australian Diabetes Educators Association 2013

Redox imbalance and endothelial DNA damage as early markers of atherosclerosis risk in prediabetes (#268)

Hayder Al-Aubaidy 1 , Herbert Jelinek 1 2 , Laura Maschirow 3 , Sarah Meidinger 4
  1. Charles Sturt University, Orange, NSW, Australia
  2. Khalifa University, Abu Dhabi, United Arab Emirates
  3. Department of Cellular Biology, University of Hamburg, Hamburg, Germany
  4. Nutrition Science, University of Potsdam, Potsdam, Germany

Objectives: This study aimed at investigating the connection between oxidative stress & oxidative DNA damage in subjects with impaired fasting glucose (IFG).
Design and methods: This is a University-based research project. Clinical data of sixty-four participants without a known history of cardiovascular disease and/or diabetes were analysed. Participants were recruited via public media announcements. Differences in the level of biochemical markers of oxidative stress (erythrocyte GSH/GSSG), and endothelial dysfunction (urinary 8-hydroxy-2-deoxy-guanosine– 8OHdG) were determined for prediabetes and control subjects.
Results & conclusion: The redox balance was significantly impaired in the prediabetes group with an increased erythrocyte GSSG (18.3±13.2 vs 25.2±13.1mg/dL) and a decreased GSH/GSSG ratio (10.6±6.7mg/dL vs 6.1±2.6) compared to the control group (p<0.05), without changes in GSH oxidation and urinary 8-OHdG. This result follows on from our previous work that indicated a complex interaction between redox balance and oxidative stress, with 8-OHdG significantly increased when GSH levels are decreasing in IFG. Increased oxidative stress as reflected by the reduced GSH/GSSG ratio is the likely link between moderate hyperglycaemia in prediabetes leading to reduced erythrocyte GSH and endothelial dysfunction. The GSH/GSSG ratio, GSH and urinary 8-OHdG offer a possible tool for the assessment of oxidative stress related vascular pathology.